The Mannitol agar exhibited a change with S. These color change occurrences are indicative of pH changes within the agar dishes. Is there a difference? In this experiment, we heat-shocked our E.
The needle is flamed before inoculation in order to prevent cross contamination of bacteria on the medium and after to kill the bacteria collected for the culture. However, only one tiny colony could be spotted at the edge of our dish.
Indication of growth within the four types of agar. Turn the plate a quarter turn and go back and forth several more times. In order to determine the efficiency of the transformation we need to determine the initial amount mass of plasmid that was spread on the plate and relate this to the number of transformed colonies that were observed on the experimental plate.
Why do we need the control plates? Choose Type of service.
If there are Ecoli lab report many to count then record "lawn". Differential or isolative media are those that are used for differentiating or isolating particular bacterial groups based upon their metabolic properties, and do so by the addition of specific indicator compounds.
Why do we need the control plates? Exposure of the cells to cold calcium chloride solution, in Ecoli lab report with the "heat shock" discussed in step 12 below, causes the cell membrane to become porous and thus the cells are made "competent" for transformation.
Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. Count how many colonies are present on each plate. Now for the most exciting dish: We successfully predicted that the transformed bacteria on the ampicillin plate would be luminescent, that no untransformed bacteria would grow on an ampicillin plate, and that untransformed bacteria would grow on an ampicillin-free plate but that it would not be luminescent.
Brock Biology of Microorganisms, 11th ed. On plate 2 the transformed bacteria can still grow to form a "lawn" since there is no antibiotic present to inhibit growth of bacteria without the plasmid.
Coli in the LB - plate. Discard this pipette into the bacterial waste. I definitely accidently put 3x the amount of transformation fluid that I was supposed to, so that is why the value is skewed. Get Access Microbiology lab report on Escherichia coli and Staphylococcus aureus Essay Sample In this lab experiment two different types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies.
This experiment was primarily for the purpose of growing E. This one had food for the bacteria and the antibiotic. Selective or inhibitory media allow for, or inhibit, specific bacterial groups based upon the addition of distinct components to the media. It is important to hold the cap of the tube in your hand, and angled downwards, because microbial contamination can occur if the cap is exposed to another surface or the open air.
Another possibility could have been that we did not spread enough pGLO which contained the enzyme to resist ampicillin onto the dish. Wash hands before leaving lab. We were obviously slightly under that range. Staphylococcus species, however, are able to grow in these conditions and S.Lab Report 2 Bacterial Transformation Jennifer Hauss March 4, Bacterial Transformation Lab Report Introduction In this lab, the goal was to transform the bacteria e-coli to glow in the dark (or under a black light Four plates were set up with agar in them for the bacteria to feed on and grow.
Lab Report of the Experiment of Conjugation of E. Coli Words Jan 21st, 9 Pages Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. Biotechnology Bacterial Transformation Lab: The effects of pGLO DNA on E.
coli Method Introduction Bacteria transformation is the process of a bacterium absorbing and integrating naked DNA located on the surface of their membrane. Escherichia coli.
After performing the Gram stain to determine that the unknown was a Gram negative rod, the organism was grown on a TSA slant for use in inoculating the rest of the biochemical tests. UNKNOWN LAB REPORT.
Unknown number Escherichia coli, Klebesiella pneumoniae, Enterobacter aerogenes, Proteus vulgaris, and Pseudomonas aeruginesa. The first biochemical test performed was a Simmons Citrate, and after observation a negative result was concluded. Activity 4: Transformation of E.
coli using green fluorescent protein. Information for Teachers Clean the lab benches with the bleach solution and remember to wash your hands before leaving the lab. Procedure. Day 1: Contact Us - Report a Bad Link.Download